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OBJECTIVE To observe the infection after ophthalmic operation in 276 patients with AIDS.METHODS This trial involved 276 patients(276 eyes) with AIDS.They were divided into 2 groups according to surgical sites:112 patients(112 eyes) were in the first group who were undergone eyelid or conjunctival operation and 164 patients(164 eyes) were in the second group who were undergone cataract or glaucoma operation.There were 325 patients(325 eyes) who did not suffered with AIDS in the control group: 130 patients(130 eyes) were in the third group who were undergone eyelid or conjunctival operation and 195 patients(195 eyes) were in the fourth group who were undergone cataract or glaucoma operation.The infections were observed after operation.RESULTS There were 30 cases(26.79%),8 cases(4.88%),12 cases(9.23%) and 8 cases(4.10%) suffered with infection after operation in four groups respectively.There were 38 infected cases(13.77%) in patients with AIDS and more than that in patients who did not suffered with AIDS(20 cases and 6.15% ?2=9.07,P
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Objective To study the changes of corneal sensitivity in diabetic and nondiabetic subjects after phacoemulsification. Methods This trial involved 42 diabetic subjects(42eyes)and 46 nondiabetic subjects(46 eyes)with cataract.All eyes were underwent phacoemulsification.Corneal sensitivities were tested before surgery and 1 day,1 week,1 month,3 month,6 month after surgery.Results The corneal sensitivity of diabetic subjects was greatly reduced at 1 day,1 week,1 month,3 month after surgery(P＜0.05),and returned to the preoperative values at 6 month(P＞0.05).The corneal sensitivity of nondiabetic subjects was reduced at 1 day,1 week,1 month af- ter surgery(P＜0.05),and returned to the preoperative values at 3 month(P＞0.05).The corneal sensitivity of diabetic subjects was lower than nondiabetic subjects before surgery(P＜0.05).The reduced range of corneal sensitivity in diabetic subjects was greater than that of nondiabetic subjects(P＜0.05).Conclusions The corneal sensitivity is decreased and the recovery is postponed after poacoemul- sification in diabetic subjects.
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Objective To study the culture method of conjunctival epithelium in vitro.Methods The rabbit conjunctival epithelium was cultured by tissue inoculation and was explanted in culture dish or on human amnionic membrane for fwo weeks.The cell morphology and growth features were observed.Results The rabbit conjunctival epithelium explanted in culture dish connected each other into membrane at eighth days around,which were monolayer cells was observed.The rabbit conjunctival epithelium explanted on human amnionic membrane connected each other into membrane at sixth day,and multi-layer cells were observed.All cultured cells showed positive in CK13 staining by indirect immunofluorescence staining.Conclusions Tissue inoculation is a good approach of conjunctival epithelium culture,and multi-layer cells can be gained if the tissue be exlpanted on human amnionic membrane.
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Objective To study the method of the cultured human conjunctival epithelium combined with amniotic membrane transplantation for treatment of conjunctival defect. Methods 20 eyes of 10 rabbits were divided into study group (10 right eyes ) and contoal group (10 left eyes ). The conjunctival epithelium got from conjunctiva of fornix of study group about 1mm?1mm was explanted on human amniotic membrane and was cultured in vitro by tissue inoculation 2 weeks before operation. The bulbar conjunctivals of 20 eyes were excised. The conjunctival defect was covered by the graft in the study group and uncovered in the control group. The growth features of the grafts were observed. Results The conjunctival epithelium explanted on human amniotic membrane connected each other into membrane in 6 days, and multi-layer cells were observed. In the study group, all grafts were inosculated with and were similar to normal conjunctival in 2 weeks. There was not immune rejection occurred. In the control group, the conjunctival defect was recovered by scar. Conclusion It is effective of the cultured conjunctival epithelium combined with amniotic membrane transplantation for treatment of conjunctival defect.